ABSTRACT
@#Ocular squamous cell papilloma is a common benign ocular tumor which has been associated with human papillomavirus(HPV)infection, ultraviolet(UV)radiation exposure, <i>etc</i>., but the pathogenesis still remains uncertain. The tumor often shows a fleshy or gray-white mass with a cauliflowerlike or spongylike appearance involving the conjunctiva, caruncle, limbus, eyelid or orbit. It can be diagnosed by combined examinations including high-resolution optical coherence tomography(HR-OCT), ultrasound biological microscope(UBM)and histopathological examination. Risk factors for local recurrence of ocular squamous cell papilloma may include location of the tumor, cellular atypia and ways of treatment. Surgery has historically been the mainstay of treatment. In addition, adjunctive therapies including cryotherapy, amniotic membrane transplantation, mitomycin(MMC), interferon α-2b(IFN α-2b)or 5-fluorouracil(5-FU)may also help to improve the local control. However, more studies are still needed to prove the effects of adjuvant therapy to surgery in ocular squamous cell papilloma.
ABSTRACT
Objective To observe the effects of Wendan decoction (WD) on depression-like behavior in a rat model of Parkinson's disease (PD) and explore the related mechanism.Methods Rodent model of PD was established by unilaterally lesioning medial forebrain bundle with 6-hydrodopamine.After intragastric administration with WD,the rats's behavior changes were detected by the open field test,sucrose preference test and forced swimming test;the contents of monoamine neurotransmitters in the rat brain were assessed by high-performance liquid chromatography with electrochemical detection.Results Compared with those of sham-operated rats,the horizontal and vertical activities of the PD model rats decreased significantly,and sucrose consumption decreased significantly,but immobility time during forced swimming was significantly prolonged.The contents of dopamine (DA),5-hydroxytryptamine (5-HT) and noradrenaline (NA) in the medial prefrontal cortex (mPFC) and DA in the striatum decreased significantly.After administration of WD for 2 weeks,the immobility time of the PD model rats was significantly decreased,sucrose consumption increased significantly;DA,5-HT and NA levels in the mPFC increased significantly.Conclusion WD improves the depression-like behavior in PD model rats,and the mechanisms may involve the regulation of monoamine neurotransmitters in mPFC.
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effects of the two-valence vaccine consisting of Helicobacter pylori (H. pylori) catalase and urease subunit UreB in preventing H. pyloriinfection in mice.</p><p><b>METHODS</b>C57BL/6 mice were divided into 7 groups and immunized with intragastric administration of catalase and UreB (both 100 microg) plus cholera toxin (CT, 2 microg), catalase (100 microg) plus CT (2 microg), UreB (100 microg) plus CT (2 microg), catalase (100 microg), UreB (100 microg), CT (2 microg), or PBS, respectively, once a week for 4 consecutive weeks. Two weeks after the last immunization, all the mice were challenged by live H. pylori, and sacrificed 4 weeks after the challenge to obtain the gastric mucosa samples for detecting H. pylori using semi-quantitative bacterial culture assay.</p><p><b>RESULTS</b>The total protection rate in mice immunized with the two-valence vaccine, single-valence vaccine of catalase, and single-valence vaccine of UreB was 83.3% (20/24), 41.7% (10/24) and 54.2% (13/24), respectively, and the rate in the other 4 groups were all 0. The H. pyloricolony density in mice with vaccination was significantly lower than that of other 4 groups (P<0.05). The total protection rate and H. pylori colony density differed significantly between the two-valence vaccination group and the single-valence vaccination groups (P<0.05).</p><p><b>CONCLUSION</b>The two-valence vaccine consisting of catalase, UreB and adjuvant has better immunoprotective effects than the single-valence vaccines.</p>
Subject(s)
Animals , Male , Mice , Bacterial Proteins , Allergy and Immunology , Bacterial Vaccines , Allergy and Immunology , Catalase , Allergy and Immunology , Helicobacter Infections , Allergy and Immunology , Helicobacter pylori , Allergy and Immunology , Immunization , Methods , Mice, Inbred C57BL , Random Allocation , Urease , Allergy and ImmunologyABSTRACT
Helicobacter hepaticus is nongastric helicobacter that can reside in the hepatobiliary and intestinal systems of many animal hosts, leading to proliferative hepatitis, hepatocellular carcinoma, typhlitis, and colonitis. In this study, the intestinal mucosa was isolated from BALB/c mice to prepare tissue homogenate and spread onto selective C jejuni blood agar plates for incubation in the presence of trimethoprim, vancomycin, and polymyxin at 37 degrees Celsius; under microaerobic conditions in vented jars containing 5% O2, 10%CO2, and 85% N2. The bacteria were identified morphologically and biochemically. Gene sequence analysis of the 16s rRNA confirmed the presence of Helicobacter hepaticus, and the success in isolating this bacteria may have significant implications for studies of nongastric helicobacter.